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We aimed to evaluate the impact of an educational intervention on the surface cleaning and disinfection of an emergency room. This is an interventional, prospective, longitudinal, analytical and comparative study. Data collection consisted of three stages Stage 1-baseline, Stage 2-intervention and immediate assessment, Stage 3-long term assessment. The Wilcoxon and the Mann-Whitney test tests were applied. We performed assessments in each stage totaling evaluations. Therefore, conclusively, the educational intervention had a positive result in the short term for ATP; however, the same rates are not observed with the colony-forming units CFU , due to their high sensitivity and the visual inspection method since four surfaces had defects in their structure.

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